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In the assay the Hoechst fluorescence is quenched by Brdu,
which results in a decrease in Hoechst fluorescence of S
phase cells during the first cell cycle.
What is unclear to me is: Why does the Hoechst fluorescence
of S phase cells increase when cells are in the second and
third cell cycles ?.
In the available literature (Methods in Cell Biology, vol
41), the authors suggest that this has something to do with
single or double strand labelling.
Could somebody please explain ?
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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