U937 & CD20

Thomas Delohery (t-delohery@ski.mskcc.org)
Thu, 27 Apr 1995 12:28:31 +0530

Hi, we're having problems with non-specific binding of anti-CD20 to
U937 cells. BD's @CD20-FITC works fine but @CD20-PE has high
background (supposedly the same clone). MAb from different
clones obtained from Caltag and Coulter also have high background
relative to isotype controls.
Can anyone make a recommendation on a MAb source?
Any help would be greatly appreciated.

tom d.

--
==============================================================================
 Thomas Delohery                        | Internet: t-delohery@ski.mskcc.org
 Manager, Flow Cytometry Core Facility  |    Phone: (212) 639-8729
 Memorial Sloan-Kettering Cancer Center |      Fax: (212) 794-4019
==============================================================================
 


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