 |
 |
 |
 |
 |
beam splitter: 460 shortpass dichroic
UV violet : 405/20 bandpass
UV green: 537/45 bandpass
Filters were purchased from Omega Optical.
Smith could verify that the filters used are adaquate for detection by removing
the beam splitter and manually switching each filter into "FL4", which I am
assuming is the "straight-through" channel. If signal is measurable, then one
could suspect alignment of the dichroic or FL5 PMT.
-Marty Bigos
Stanford Shared FACS Facility
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web