reply to K. Weber re Concentration Calibration Curves
James Leary (JLEARY@BEACH.UTMB.EDU)
Mon, 10 Apr 1995 08:42:58 -0500 (CDT)
I've done many experiments similar to this to calculate instrument deadtimes
and to check queueing theory models for cell arrival statistics. The data
you have can be used to calculate your instrument deadtime using either
simple instrument deadtime equations or by Poisson statistics. For a
detailed discussion of this see Methods in Cell Biology vol. 42: pp. 331-
358, 1994.
Incidentally these experiments are one way to keep the instrument
manufacturers honest about sample processing rates. Don't take their word
for it. Test it yourself. One caveat - sample processing rates can depend
on how you are running your experiment. Instrument manufacturers usually
quote rates which may be entirely truthful for what they tested, but those
results may mean nothing for your experimental conditions!
-------------------------------------------------------------
James F. Leary Professor of Internal Medicine
Director, Molecular Cytometry Unit-Route 0841
University of Texas Medical Branch
Galveston, Texas 77555-0841
Tel: 409-747-1930 Fax: 409-772-6527
email: jleary@beach.utmb.edu
-------------------------------------------------------------
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
