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There have been a few postings asking about RNA recovery from
flow sorted cells. This could be pretty embarrassing, but I
have to ask, has anybody tried a method we published way back
in the MolBiol dark ages of 1989 (Cytometry 10:199-204)?
Our research priorities shifted very soon after we developed
the technique so I don't have much more experience than what
the manuscript describes, but at the time I thought it was
pretty cool (cool=sensitive, linear, relevant). It's easy,
seemed to work consistently and over a nice dynamic range. I
was sure it would revolutionize the field (I often have that
effect), but I haven't seen squat.
Any experience? If the truth is too brutal you might send me
a note directly, but I have always wondered.
Thanks, Jack.Dunne@Syntex.com
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
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Phone: (765)-494-0757;
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