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Paranoia of RNAse's will help ensure good results. DEPC-treated sheath fluid, as
well as vigilant sample-tubing cleaning are a must.
See Cytometry 11:869-874 (1990).
(They used fixed cells, 5XSSC instead of the standard PBS and washed
sample-tubing with 10% Hydrogen Peroxide.)
I'm also believe that ALL responses to interesting questions should be posted to
THE LIST.
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* Dennis J. Young Voice : (619) 543-3928 *
* Flow Cytometry Core Facility FAX : (619) 543-7487 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web