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What is the precision of the staining and how many replicates do you prepare
per experiment?
Flow cytometry *IS* limited in sensitivity (to about 5,000 FITC per particle)
and may not be the appropriate assay system. Positive/negative for fluorescence
does *NOT* mean that the antigen is or is not there!
Do you have this:
Flow Cytometry Titration of Retroviral Expression Vectors: Comparison of Methods
for Analysis of Immunofluorescence Histograms Derived from Cells Expressing Low
Antigen Levels, Sladek and Jacobberger, Cytometry, 14:23-31 ,1993)?
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* Dennis J. Young Voice : (619) 543-3928 *
* Flow Cytometry Core Facility FAX : (619) 543-7487 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web