(no subject)
Nicholson, Janet (jkn1@CIDHIV7.EM.CDC.GOV)
Tue, 21 Mar 95 13:49:00 EST
To Jim Henthorn: From a number of sources we know that formaldehyde or
paraformaldehyde will inactivate HIV-infected cells. In my experience, the
number of infected cells (and HIV copies/cell) in the cell preparation
(infected cell line vs. whole blood from HIV-positive donor) determines how
much fixative for how long. To completely inactivate a cell line with 100%
positive cells at a high copy number, I recommend overnight fixation in 1%
formaldehyde or paraformaldehyde in the refrigerator. For whole blood, 1/2
hour under the same conditions is probably O.K. The biggest issue with
sorting cells comes when the cells are not fixed.
Jan Nicholson
Centers for Disease Control and Prevention
Atlanta, GA
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