RE: PerCP and the Vantage
Phillip McCoy (pmccoy@UMDNJ.EDU)
Wed, 15 Mar 1995 14:46:19 -0500 (EST)
We have had extensive experience with PerCP and the Vantage using
an Enterprise laser. We find it is very useful at all outputs of the
Enterprise, although we usually run ours at 50-100mW 488. I appreciate
your concern as I have recently had a discussion with Carl Stewart on
this issue. He believes that the only reason we can run PerCP at higher
laser power is that our instrument is not properly aligned. I disagree,
as the CVs and intensities of our other fluorochromes indicate that all
is well and our power outputs are in the ballpark. I certainly am not a
big believer of the "high laser power bleaches PerCP" theory. At any
rate, I recommend you do what works best for you.
Phil McCoy
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
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