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I just want an idea of what is the best I can expect to achieve.
For instance, I routinely obtain full CVs less than 2% on green,
orange and red fluorescence with Coulter's "DNA Checks" with the
1st laser. If the 2nd laser is tuned to 488 or 514nm (same power
and optical filters) what is the best I can hope for? CVs of 3%? 8%?
Before the dismantling I was looking at CVs around 15%!!!
Any response would be greatly appreciated.
tom d.
"At the heart of the slaughterhouse - always - enough room to nourish awe."
- J. Nichols
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 ==============================================================================
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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