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Aki:
Way back in Grad School, I was using an Epics V and was also trying to
examine cell cycling in T cells. I tried using the Hoechst (sp?) 33342 (also
maybe the 33258, but I really do think it was the 33342 - even after 9
years!). Needless to say, I was unable to get a classic growing cell cycle
histogram with Hoechst stains. THE only thing I did accomplish was being
able to roll the Epics in UV mode in about 20 minutes - that was all.
To complete the project, we wound up using Propidium Iodide via a fixation,
triton, RNAse like protocol. That gave us the picture purfect curve we
wanted to see...
Hope this helps,
David
>Aki Hoji (wondering when spring comes here)
>University of Wisconsin, Madison.
*wishing* for big snow but dissapointed at yet another wimpy mid-west
winter here in STL!
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web