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As most of our work is in immunology, this model system works well for us.
However, every cellular system seems to apoptose a little differently. I
have especially found differences in the kinetics of apoptosis. Some cells
die very quickly, some take longer. So how this model will apply to your
kerotinocyte system is unknown to me. I have also found that some apoptosis
detection methods described in the literature are not appropriate methods
due to the treatment of the cells to induce apoptosis. For instance, a
method that utilizes changes in membrane (HO342, 7AAD) cannot be used in
systems where cells are stimulated with PMA and ionophore.
Anyway, good luck and have fun.
Julie
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Julie A. Auger voice: 312-702-9212 lab
Director, Flow Cytometry Facility 312-702-9261 office
University of Chicago fax: 312-702-3701
5841 S. Maryland Ave. MC-1089
Chicago, IL 60637 e-mail: jauger@flowcity.bsd.uchicago.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web