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If we washed more extensively/longer, would it help lower non-specific,
cytoplasmic
binding (although we do have 1% BSA in all of our incubation steps) and
maybe make the nuclear staining more prominent?
Thanks again.
ray
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Raymond B. Hester "We want only to show you
Flow Cytometry Lab something we have seen and to tell
The University of South Alabama you something we have heard...
Mobile, AL 36688-0002 that here and there in the world
Email: rhester@jaguar1.usouthal.edu and now and then in ourselves
Voice: (205)460-6029 is a New Creation."
- Paul Tillich, The New Being
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web