file compatability

Wendy Jones (wjones@uhunix.uhcc.Hawaii.Edu)
Thu, 9 Feb 1995 16:11:22 -1000

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I'm having a file compatability problem that hopefully, someone out there
in the net can help me with.

A short time ago I had the opportunity to train in a flow lab on the mainland
(I'm in Hawaii). During my training I ran plant nuclei stained with PI
for DNA content analysis. The instrument was an EPICS, and histograms
were generated using ACQCYTE software (Pheonix) on a PC. The files were
saved in lis mode, not as histograms. NOw that I'm back home I'd like to
do something with those files. Hence my problem. The available software
here is mcycle or cytocell, neither of which appear to like my files.

Any suggestions? (besides start over)
The files are in FCS 1.0 format (ortho not MADS, if thats of help)
the parameters were LFL,FL and FS.

Thanks,
Wendy

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu