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Thanks for the references. I assume the user to whom you refer is doing
the F-actin by flow? What are the advantages of NBD over FITC?
I know FITC is very pH sensitive around neutrality; maybe NBD isn't?
Is NBD as bright as FITC? Can it be excited with argon 488?
Do you have a preferred procedure for permeabilization, stabilization and
staining? In the literature, we've seen sucrose/Mg/triton,
paraformaldehyde with lysolecithin, and maleimidobenzoyl
N-hydroxysuccinimide ester/triton + formladehyde; hope we don't need to
characterize all three ourselves.
In message Mon, 06 Feb 1995 09:10:46 -0500 (EST),
cheryl@immune.med.utoronto.ca writes:
> One of the users at this facility has done quite a lot of work on F-Actin
> assembly in neutrophils using NBD-phallacidin. I've included references
> that I had from our last grant application, but they are not that recent.
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
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