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Are any pan leukocyte markers available for turkeys? Will the RBCs stain
differentially with DNA dyes? Has anyone used the Cytek absolute count
system with a FACScan?
To start with, I am thinking of the following experiment.
1. Fix peripheral blood with 1% glutaraldehyde/PBS, at 4 degrees C
overnight.
2. Aspirate fixative and resuspend in PBS with 30 Ug/ml SDS to sphere RBCs.
3. Run samples and look for light scatter clusters.
Am I anywhere near the ball park here? Thanks for your comments.
Regards,
David Abbott
DL.Abbott@Lilly.Com
P.S. My ISAC directory internet address is wrong. Please substitute a
"L" for the "K".
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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