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In the past we have sorted reticulocutes stained with thiazole orange
on a FACS440. There were similar problems to those you are aware of
with acridine orange.
We reserved a complete nozzle holder, nozzle and sample line for
thiazole orange work as we found this only took a few minuets to
swap over and guaranteed that no dye was lurking in the system.
Our decontamination procedure for thiazole orange and acridine
orange on our FACScan and FACS440 is 10 mins. ethanol to dissolve
any residual dye and 10 mins. 7X to remove any cellular debris.
We do not use any bleach.
Hope this information is useful to you.
Terry Hoy
Department of Haematology
University of Wales College of Medicine
CARDIFF CF4 4XN
UK
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web