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> Hi,
> I'm new to this list, so I hope these are appropriate questions:
>
> Does anyone know where I can obtain an antibody to the human IL-8 Receptor
> for use in Flow cytometry?
>
> If I can't find an antibody, I have an alternate idea:
> 1. incubate cells with IL-8
> 2. Incubate cells with polyclonal Rabbit anti-IL-8 antibody
> 3. Incubate cells with with FITC-conjugated Goat anti-Rabbit antibody
> --Does anyone have any experience with this type of approach to label any
> receptor molecule?
>
> Thanks in advance.
> --Rich
I don't know where you can find the anti-IL-8R but your alternative idea
should work. You may want to biotinylate the IL-8 and use a FITC-avidin
conjugate, if you can. I think Chris Goodnow (Stanford) has done something
similar with HEL-specific B-cells.
Todd Armstrong, UMBC.
> Richard Moldwin, M.D., Ph.D.
> Assistant Professor of Pediatric Hematology/Oncology
> University of Chicago Hospitals
> 5841 S. Maryland Ave., M/C 4060
> Chicago, IL 60637
>
> E-mail: rmoldwin@midway.uchicago.edu
> Phone 312-702-6808
> Fax 312-702-9881
>
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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