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Dave Coder
dcoder@u.washington.edu
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Date: Mon, 05 Dec 1994 10:21:00 -0400 (EDT)
From: James Weaver 202-205-5037 FAX 202-205-4940 <@VM.CC.PURDUE.EDU:WEAVER@FDACD>
Subject: Archive of Spectra?
To: CYTOMETRY@flowcyt.cyto.purdue.edu
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Posting-Date: Mon, 05 Dec 1994 10:52:00 -0400 (EDT)
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At the recent ISAC meeting there was discussion of the archives that are being
established at Los Alamos for flow cytometry files. These currently include
examples of various types of data files to aid software developers.
I would like to propose that these archives be extended to include
excitation and emmision spectra of the various fluorochromes used in cytometry.
I would suggest that these be normalized to allow easy comparison and overlays
to evaluate compensation needs. I have added a possible file structure below but
I am very open to suggestions, additions, modifications etc. I would also like
some discussion on such issues of checking the calibration of
spectofluorimeters and measurement of fluors that need enzymatic processing (AM
esters). I am in the process of collecting some spectra that could be used to
start things off but contributions from others will be welcome once the format
is set. Thanks for your interest.
Jim Weaver
Division of Research & Testing
ORR, CDER, FDA
Laurel, MD
V: 301-594-5879 F: 301-594-3037 E: WEAVER@FDACD.CDER.FDA.GOV
[ Example of Fluorescence Spectrum Header]
Fluorescent Molecule: Rhodamine 123
Source: Sigma
Catalog #: R-8004
Concentration: 5.2 nM
Conjugated to: None
In: Water
Instrument: MacPherson
Collected by: James L. Weaver
Address: FDA, CDER, MOD-1
Address: 8301 Muirkirk Rd
Address: Laurel, MD 20708
Phone: 301-594-5879
FAX: 301-594-3037
Email: WEAVER@FDACD.CDER.FDA.GOV
Date: 12/5/94
Emmission Spectrum, Excitation at : 488 nm
nm Normalized Signal
505 0.322
506 0.365
etc
699 0.001
700 0.000
Excitation Spectrum, Emmission at: 525 nm
nm Normalized Signal
400 0.001
401 0.000
etc
504 0.911
505 0.873
End of File.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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