Elite ESP
Rehse, Mark (REHSEMA@cellpro.cellpro.com)
18 Nov 94 09:29:03 PST
This is in response to K. Webber's poll concerning the ESP upgrade. I
have been sorting with the ESP upgrade for about 6 months. We upgraded
our 3 year old Elite when the need to sort rare events became a time
constraint. For the first few months most sorts were a disaster and I
considered retrofitting the old signal processors. However, after going
through the hoops with Coulter and trying matched sets of enclosed
bimorph/flow cell body/flow cell tip QCed in Florida and replacing a
noisy power supply sorting performance began to improve from a "no sort"
condition to 80-90% purity. Several tricks I learned- A) I leave the
sorter powered up 5 days/wk 24 hours/dy with screens dark, fluidics
powered down, sort crystal at 0 % until several hours before a sort. B)
This particular configuration takes 30kHz, 75% Drive, 100% defl., 12 lbs
sheath, drop delay=43.0, sort mode=purity. Drop delay profile comes up
with 18-20 beads/20 beads sorted in drop 43, 0-1 in 42 and 44. Eight to
nine/10 phase settings good. At these settings any change in drop delay
will compromise purity and recovery and so needs almost constant
monitoring. Occasionally a small movement of the aligator clip where it
conects to the flow cell body will "correct" some fanning side streams.
We regularly sort at 7-8,000 events/sec and get 95%+ purities at these
settings. Your particular machine may require slightly different settings
but keep working with it. Hope this helps.
Mark Rehse Rehsema@cellpro.cellpro.com
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
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