Re: Sorting Biohazards--second try

Susan D. DEMAGGIO (SDDEMAGG@uci.edu)
Wed, 16 Nov 94 08:22:00 PST

Steve, I actually did this very thing - sorting a population positive for the
expression of a particular protein of interest tagged with FITC. We have an old
741 EPICS and just kept the flap on the sort chamber closed - sprayed the
chamber out with alcohol before and after and did the normal bleach and alcohol
washes of the lines before and after. No one died!! so I think we did o.k. I
would like to hear if we were just lucky, or do others do this type of "foolish"
thing??

Sue DeMaggio

______________________________ Reply Separator _________________________________
Subject: Sorting Biohazards--second try
Author: (Steve G. Hilliard) <hilliard@zookeeper.zoo.uga.edu> at biosmtp
Date: 11/14/94 8:48 PM

I've had trouble "following my bouncing mail" (can't sing anyway ;-))
but I get the feeling my first attempt didn't make it. To reiterate, I
have a user who wants me to SORT antibody-labelled, VIABLE _T. cruzi_. I'd
appreciate anyone's thoughts--current regulations (if any), precautions,
"don't even consider it", etc. Do we need to put our Elite in a P2 room,
or just forget it entirely? Anyone?

Thanks,
Steve
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Steve G. Hilliard, Cell Analysis Facility |
University of Georgia | "Be good and you will
hilliard@zookeeper.zoo.uga.edu | be lonesome..."
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