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> DOES ANYONE KNOW OF A SUPPLIER FOR EUROPIUM CHELATES SUITABLE FOR
> CONJUGATING TO ANTIBODIES. WE ARE INTERESTED IN USING THESE REAGENTS FOR
> TIME RESOLVED FLUORESCENCE IN FLOW FOR DETECTING VERY SMALL PARTICLES.
>
> HAS ANYONE GOT EXPERIENCE WITH TIME RESOLVED FLUORESCENCE IN FLOW.
>
> ANY SUGESTIONS FOR FLUOROCHROMES WITH LONG FLUORESCENCE LIFETIMES.
> REGARDS,
Guenter Valet has published data on flow cytometry using europium
chelates at least in abstract form I can't remember if it was
published elsewhere - also Seveus published a paper in Cytometry
13:329 (1992) on imaging and time resolved fluorescence.
Paul Robinson
J.Paul Robinson, Purdue University Cytometry Labs
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web