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We have used TO-PRO-3 iodide (TP3) as an alternative for
propidium iodide in order to measure DNA content. In this way it
is possible to combine DNA measurements with the analysis of
the binding of two antibodies against cytoplasmic or nuclear
antigens (indirect immunofluorescence with FITC and PE). These
experiments were performed on an Elite with 488nm excitation for
FITC and PE and 633nm excitation for TP3. The technique is
described in the last issue of Cytometry (17:185-189, 1994).
Piet van Erp tel: +31 80 613548
Department of Dermatology fax: +31 80 541184
University Hospital Nijmegen e-mail: P.vanErp@derma.azn.nl
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web