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Does anyone have any tips on how not to lose cells after
ETOH fixation . I have heard from a number of our users that they
start out fixing 2x10E6 cells/tube and see a pellet after the
first spin, but after a long procedure (like Oncor Apoptag) they
seem to have almost nothing left. They use polypropylene tubes, and
have notice a slight increase in retained cells by using FCS in the
wash media, but the numbers are still terrible. Im pretty sure the
loss is in the centrifuge steps- any suggestions?
Peter Lopez
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Peter A. Lopez (617)632-3179 (voice)
Dana Farber Cancer Institute (617)632-3139 (voice)
Core Flow Cytometry Facility NO FAX!
44 Binney St. Room J-312
Boston,MA 02115 PLOPEZ@sorter.dfci.harvard.edu
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web