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The method involves the use of thermal treatment (75 degrees for 90 min) to
reverse the effect of formalin on the binding of PI to DNA.
Has anyone else repeated this procedure with similar results.
My first attempt gave only a slight increase in fluorescence intensity.
Any suggestions?
Thanks, joe
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Joe Lima
jlima@ftzsmmns-amedd.army.mil
Fitzsimmons Army Medical Center
Aurora, CO USA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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