Generating TIME on BD HP's

Geoffrey Osborne (Geoff.Osborne@anu.edu.au)
Thu, 1 Sep 94 10:25:00 EST

Mike,
regarding the following . . .
>Eric,
>
>FACScan Reseach Software and FACStar Research Software do not record TIME as a
>list mode parameter; LYSYS II is the only application from BD on the HP that
>does generate time. LYSYS II can be run on a CONSORT 30 Color system.
>
>For files generated by software that does not generate time separately, most
>applications extrapolate time by using the $BTIM and $ETIM keyword values to
>get the total time, then divide by the number of cells indicated in the $TOT
>keyword value.
>
>Best Regards,
>Mike Lance

I'm suprised that you place such faith in being able to utilize the $BTIM
and $ETIM field in LYSYS II generated list mode data to calculate "time".
You may recall that some time ago I emailed you regarding an occassional
error in these fields, which Eric is also aware of since he has dealt with
some of these files. Since that time others, such as the article I'll
include, have mentioned similar findings. I'd recommend treating this method
of generating time as a parameter with some caution . . .

====================== Include starts here ===========================
Date: Mon, 16 May 1994 14:40:54 -0300 (ADT)
From: Bill=K=Li%BIOBOD%DFOSF@sfnet.dfo.ca
Subject: Nonsensical $BTIM and $ETIM
To: cytometry@flowcyt.cyto.purdue.edu
Message-Id: <01HCESH7SY4I00A1AJ@BIONET.bio.dfo.ca>
Content-Transfer-Encoding: 7BIT
Status: RO

On occasion, I get nonsensical values for the keywords $BTIM and $ETIM (begin
and end times for acquisition). My listmode files are created by the Becton
Dickinson LYSYS-II version 1.1 software under HP Pascal 3.22. The format for
these keywords should be hh:mm:ss. In some of my files, I note that the
value for "ss" (seconds) exceeds 60.
Can anyone offer advice on how to prevent such occurences?

----------------------------------------------------------
W. Li
Biological Oceanography Division
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada B2Y 4A2

email:
Bill=K=Li%biobod%dfosf@sfnet.dfo.ca

========================include ends here =================================

regards,
Geoff.
=====================================================================
Geoffrey Osborne | ____ __ o Ahh!
Flow Cytometry (FACS LAB) | __ `\ <,_
John Curtin School of Medical Research, | __ (*)/ (*)
Australian National University, | ==============|
CANBERRA, AUSTRALIA. | |--|
Email: Geoff.Osborne@anu.edu.au | |--|...
======================================================================


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