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>
> I would like to know how much UV laser power people are finding necessary for
> calcium flux using "indo" staining.
>
> I am interested in knowing what UV power is being used on the Facstar Plus,
> Facs Vantage as well as the Coulter Elite.
On our FACStarPLUS we have a Coherent 90-5. It's an older laser design
(not quite the controls as on current models), but very solid.
We've used 150 mW of uv for indo-1; this power output draws 36.5A and has
increased only 0.5A in three years (it's used in uv about 3 times a
week). The FACStar is, of course, a jet-in-air system. A system with a
cuvette will require less power.
With Hoechst, we get good resolution (cv's on thymic cells can
be less than 3%).
Dave Coder
University of Washington
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web