Alignment Fluorospheres
Rehse, Mark (REHSEMA@cellpro.cellpro.com)
12 Aug 94 08:37:37 PDT
Dear Fellow Flow Experts,
I am searching for a manufacturer of fluorescent beads. These beads must
be 6-8 um in diameter, fluoresce under 488 nm excitation and emit from
525 to 675 nm for detection in the typical FL1, FL2 and FL3
configuration, and, here's the catch, have a fluorescence intensity
similar to that of lymphocytes; about 1000-2000 MESF.
Coulter Diagnostics sells Immunobrite beads as a kit which contains
level II beads. These are perfect for my applications, however, they only
sell these as a kit. To get one 5 mL bottle one must purchase the entire
kit containing blanks plus 4 other fluorescence intensity standards. This
is too expensive.
If anyone knows of such a product could you please send the
manufacturer's name and how they can be contacted. Many thanks in
advance.
Mark Rehse
Rehsema@Cellpro.cellpro.com
(206) 489-8850
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu