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I was wondering what the "absolute" CV of fluorescent microspheres might
be? When we employ beads for alignment using flow cytometry, we measure a
population CV (pulse height or integral) which presumably reflects a
combination of the intrinsic error of the instrument as well as the error
inherent to the beads themselves. Does anyone have any ideas as to the
relative contributions of each of these factors to the measured CVs? What's
the lowest CV anyone has obtained with fluorescent microspheres?
I look forward to your responses.
David Galbraith
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web