Re: fluorescent dyes

Ivan Shaw (IVAN@MNI.LAN.McGill.CA)
Mon, 21 Mar 1994 09:35:41 EST5EDT

> This may sound kinda crazy, but I have been doing this awhile and it
> seems to work. If you mix cells (viable) with trypan blue and excite at
> 488 nm, believe it or not, they emit in the red region (645). If you
> look at these cells on a microscope with appropriate excitation, they
> emit a bright red ring fluorescence. And, if the cells are dead, you
> guessed it! They take up trypan and go from the red ring to full red
> cell fluorescence.

Dr. Longley,

This is a really neat trick that you have here. Would it be possible
to know the concentration of Trypan that you use for each cell
sample? Thank you.

- ivan

------------------------------------------
Ivan Shaw "The Angel of Death"
Neuroimmunology Unit
Montreal Neurological Institute
Montreal, Canada H3A 2B4
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Internet e-mail: Ivan@MNI.LAN.McGill.CA
Telephone: (514) 398-3002
FAX (514) 398-7371

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu