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A neighboring institution just asked for our opinion regarding the use
of the Ortho Cytoron Absolute for multiparametric DNA analysis
in cultured carcinoma cell lines. While I am aware of some of the
pro's and contra's of the use of the Facscan and Profile II for DNA
analysis, I do not know anyone who is doing the same type of
analysis on a Cytoron.
The Cytoron seems to have a rather narrow laser beam (5 um in one
direction). The instrument has an integrated pulse processor for the
red fluorescence channel. S-phase analysis includes the
"Dean"-model.
My question is, whether anyone can report some practical
experience about combined DNA and immunofluorescence analysis
on the instrument.
Thanks for any comments
Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, F.R. Germany
Tel. +49 (941) 944-6208, or -6233
Fax +49 (941) 944-6202
Internet: GROTHE@ALF1.NGATE.UNI-REGENSBURG.DE
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web