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About your Q-Prep question, in my experience with it, I cannot say that I
ever saw a difference in fluorescent intensities as compared to my
non-Q-preped samples (on facsan, Cytofluorograf or profile instruments)
however, I did fix my non-Q-preped samples with paraformaldehyde which
could make an intensity difference if you are not doing the same since
immunoprep does have 2%paraform. in it. I'm sure that you have already
asked your Coulter tech-rep, but if not, see what they have to say.
Sorry I don't know anything about buffalos or white rhinos, but I hope
this was a little help.
Margie Becker
Phoenix Flow Systems, San Diego, CA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web