Cell cycle stats in formalin fixed tissue

CJGROS@ccmail.monsanto.com
Wed, 09 Mar 94 16:04:14 CST

We are requesting help in the resolution of a technical problem. We are
attempting to study cell cycle kinetics using nuclei extracted from
formalin fixed-paraffin embedded rat liver tissue. The method works well
and good quality histograms are generated. The problem is that the
estimated S-phase in normal livers is 10-15 percent using B/D's RFIT software.
In fresh, ethanol fixed liver tissue the S-phase is 1-2 percent. So the
questions are:

1. What is the most likely reason for the disparity in the data ?

2. Is it appropriate to use the S-phase data from formalin fixed liver nuclei
as a control value in spite of the fact that it is higher than reported values
for fresh tissue?

Thanks for your help.

Cindy Gross/Dennis Flaherty E-mail: cjgros@ccmail.monsanto.com
Monsanto EHL
St. Louis, MO
Phone: 314/694-7948
FAX: 314/694-7938

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu