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> Has anyone had any luck cloning directly on a slide for FISH analysis?
> If so, what are you using for sheath fluid? We routinely use an isotonic
> sheath fluid and expect this would leave too great a salt deposit. Any
> suggestions welcomed.
>
> Thanks,
>
> Geri Pingul
> Flow Cytometry
> Fred Hutchinson Cancer Center
>
>
>Let me clarify what we would like to do. Sort cells onto a slide to be
used as a target for FISH analysis. We have a very high cell loss
when sorting into tubes and following with a cytoprep and are looking for
an improved procedure.
Thanks again,
Geri
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web