Double staining- Intracellular
Ayala Sharp (lssharp@weizmann.weizmann.ac.il)
Wed, 26 Mar 1997 08:47:10 +0000
>Dear Ayala
>I understand that there is an Internet site in which FACS questions can be
>asked.
>Would you be able to post this message for me?
>Thanks.
>Danny
>
>Subject: Double staining- Intracellular
>
>Can someone help me?
>I need to double stain for two intracellular markers:
>BrdU (nuclear) and an intermediate fillament (GFAP).
>
>I cannot use FITC and TEXAS red as conjugates, as I have no access to
>a dual laser FACS. BUT, I have been warned that PE and Tricolour may not
>enter the permeabilised cell due to their large size. Any suggestions
>will be appreciated.
>Thanking you.
>
>Daniel Harari, PhD
>The Weizmann Institute
>UserID: LCHARARI@DAPSAS1.WEIZMANN.AC.IL
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
