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I have bought some Immunotech Cyclin D1 (Cat No 1740) and tried the method
we use for Ki-67 and cytoplasmic CD3 and CD22: Acetone fix of cytospins
followed by standard 2 stage immunofluorescence. We are testing the
antibody at 1/10 and 1/100 to start with.
Several problems:
We haven't seen any convincing positivity although we have had several
possible Mantle cell lymphomas.
Also what sort of staining is seen - is it similar to TdT or Ki-67?
While we wait for a positive to turn up perhaps someone who has had
experience with this antibody in the context of B LPD could advise if the
method is inappropriate.Should we try flow?
Many thanks
Jan
Jan Nelson
Molecular Haematology
Department of Molecular Medicine
The University of Auckland
Phone: (+64)(9) 373-7599 ext 6381
Fax: (+64)(9) 373-7292
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web