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I was wondering if somebody has experience detecting fusionproteins with a
"flag" epitope, using a commercial available anti-flag antibody for
intracellular expression...
I haven't tried it, but would like to know if this could possibly work,
especially for somebody who want's to analyse cellcycle expression of a
transfected protein using a flag-fusion construct.
Any details on which mAb to use, minimal expression levels, staining
procedures would be very wellcome.
Thank you very much,
Matthias
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Matthias Haury Flowcytometry Dept Immunology Institut Pasteur
mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web