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does anyone have experience sorting fibroblast cell lines?
i'm sorting FLST cell line. this is an embryonic cell line which supports b-cell development.
unfortunately after sorting the cells remain alive but don't attach like they
normally do
i detach the cells by cell scraping and filter through a mesh post two step staining.
cytometry is on a facsvantage, 70 micron nozzle, sheath p.s.i is 11.
any ideas for increasing viablity/adherence would be greatly appreciated.
frank isdell
flow cytometry lab.
the rockefeller university
1230 york ave
ny
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web