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I've just been approached by someone wishing to look at RNA expression using
flow cytometry. He wants to do 2-colour analysis if possible and already has
his probes made up. What would be the best approach (We're using a FACScan -
488nm) ? I think his probes are DIG-labelled, so we might try to use an
anti-DIG antibody labelled with FITC or PE.
Does anyone have any basic protocols or ideas that we might be able to use,
as RNA probes and analysis by FACS is a new and exciting approach over here.
Thanks in advance
Julia Philips
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Julia Philips
Institute of Dental Research
2 Chalmers St
Surry Hills
NSW 2010
Australia
PH: 61-2-9282 0358
FAX: 61-2-9282 0368
email: jphilips@extro.ucc.su.oz.au
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web