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My colleague and I would like to set up a test for the expression of
cytoplasmic IgM for differential diagnosis of B-ALL and would
appreciate any comments /advices /references on the problems that we
encountered, mainly how would one tell the difference between a surface IgM
versus a cytoplasmic IgM? Can I somehow block the staining of surface IgM
or can I quench the surface staining as is being done with phagocytosis
tests? And is there any good controls for the tests?
We are also evaluating the possibility of performing a cytoplamic CD3
staining for diagnosis of T-ALL and, quiet possibly use it for monitoring
of minimal residual disease. We have the same concerns for this
test as we mentioned for cytoplasmic c-mu and would appreciate any comments
/advices /references.
Warm regards,
Austin Lin
Core Facility Laboratory-Flow Cytometer,
Cheng Gung Medical College
Tainan, Taiwan
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web