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We are trying to detect induced apoptosis with Annexin V and DiOC6 staining
followed by FACScan analysis. Until now, our results are not satisfying-
Staining with rhodamine works well.
Does anyone know a working protocol (apart from any datasheet or catalog
information!) and/or has experience with these staining methods? Please
reply directly to my Email
Thanks in advance
Christian Stoeckigt
Immunology Laboratory
Institute for Hemostasis and Transfusion Medicine
Heinrich- Heine University Medical Center
Duesseldorf, Germany
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web