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We recently started doing intracellular
staining for cytokines on a FACScan with CellQuest
and are trying to resolve three issues.
1) We see substantial IL-2 and IFN-g positive
cells. Is there a rational, operator-independent
way of setting the markers so that the number of
positives can be identified? Is there software
that will do this?
2) There are a few IL-4 positives and I am not sure that
I want to trust those low percentages? Is there a
better way to detect Th2 cells?
3) Does anybody have experience staining for TNF-beta (or alpha)
or IL-10?
Thanks in advance for any feedback.
Murali Ramanathan
Pharmaceutics
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web