Calcium flux markers
Surgical Labs Resident (SURGLAB1@is.rvh.mcgill.ca)
Wed, 15 Jan 97 08:36:00 PST
Dear Flow-ers,
Does anyone know of a good 3rd colour marker for detection of calcium
flux on lymphocytes, without having any leakage into Fl1 and Fl2? Right now
we are using a marker called fluo3 and it really bleeds into the other
colours. We are using a single laser FACScalibur.
Also, for people sorting on the FACScalibur, is there any way to
increase my cell recovory? Right now it is at 10%. Is it more efficient to
use a cell culture insert than a filter memrane( where I think some of the
cell loss is occuring).
Thanks in advance.
Raffi Manoukian
Royal Victoria Hospital
Montreal,Quebec, Canada
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
