Flowtec.ppt Description 1. Mechanics of a flow cell 2. How foward angle light scatter is collected 3. How 90 degree light scatter is collected 4. Fluorescence detection in the flow cell 5. Fluoresecence activated cell sorting in the flowcytometer 6. Optical filters - an example of a dichroic filter 7. Mechanism of a band pass filter 8. Examples of long and short pass filters 9. Diagramatic representation of the optic layout of a flow cytometer 10. Common excitation and emission spectra of various fluorescent probes 11. Excitation and emission spectra of propidium iodide 12. Excitation and emission spectra of FITC 13. Excitation and emission spectra of PE 14. Excitation and emission spectra of APC 15. Diagram of typical research cytometer (Coulter 753) 16. Diagram of typical clinical cytometer 17. Diagram of microscope and its optics