Instrumentation
PASIII Flow Cytometer and Cell Sorter (Partec, Münster, Germany)
Instrument Setup
- 5 photomultiplier channels
- 1 electrical sizing channel
- quarz objective and excitation light condensor
- incorporated air cooled 40mW argon ion laser (488nm)
- incorporated high pressure mercury arc lamp (HBO100)
- quarz fiber coupled 40mW HeCD laser (325/445nm)
- quarz fiber coupled 5W/100mW argon ion laser (488,514,354nm + other lines)
- glass fiber coupled 10mW HeNe laser (633nm)
- quarz fiber coupled 20mW KrFl excimer laser (254nm)
Analysis Parameters
- cell volume by hydrodynamically focused electrical sizing
- forward (FSC) and sideward (SSC) light scatter
- three fluorescence channels
- time channel
Sorting Capacities
- closed fluid piezo sorting chamber for simultaneous FSC/SSC/three fluorescence
cell sorting
- complex, non rectangular multiparameter window sorting
Data Display and Recording
- up to six parameter FCS1.0 or FCS2.0 list mode
- various one and two parameter display modes with plotting on cytometer computer
- network data transfer into DOS computers for multiparameter list mode analysis
and self learning data classification
For problems or comments, please contact:
G.Valet E-mail: valet@vms.biochem.mpg.de,
Max-Planck-Institut für Biochemie, Am Klopferspitz 18a,
D-82152 Martinsried, Germany,
Tel: +49/89/8578-2518, -2525, Fax: +49/89/8578-2563
INTERNET address: http://www.biochem.mpg.de/valet/cellbio.html
Last Update: Feb.26, 1996