Specialty Training & Education Program

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DNA Analysis

Flow Cytometry Laboratory Services
Cancer Therapy & Research Center
San Antonio, Texas 78229 
Laboratory Profile

Laboratory Manager: Steven K. Koester, M.S.

1. Facilities: 725 square foot instrument and preparation laboratory, several small multiuse rooms for tissue culture and freezer storage, office space for visiting scientists.

2. Instrumentation:
Flow cytometry: Coulter EPICS ELITE with Innova 300 argon laser and air-cooled argon. Also Coulter EPICS 752 with two 5-watt argon lasers.
Other major instrumentation: Coulter ZM cell counter, fluorescent photomicroscope.
Computers & analytical: Coulter ELITE and MDADS computers at cytometers. Also a Hewlett Packard Vectra RS/20, 80386 off-line computer with ETHERNET for EPICS 752. Software for cytometry includes Phoenix MULTICYCLE AV and Verity MODFIT.

3. Specialization Areas: Our laboratory has experience with DNA content and cell cycle measurements; DNA content plus surface marking epitopes; immunophenotyping of AIDS.

4. Special Opportunities: Tissue culture and solid tumor preparation, staining, evaluation on the flow cytometer, and cell cycle analysis. Staining of whole blood or Ficol prepared mononuclear cells for phenotypic expression. Training for EPICS 752 and EPICS ELITE applications.

5. Special Courses Offered: The Annual San Antonio Flow Cytometry Forum (7th year) which consists of 1-2 days of symposium/workshops. Our laboratory provided a workshop on cellular DNA content and S-phase analysis consisting of cell/nuclear preparation, staining, flow cytometry analysis, and cell cycle compartment analysis.

6. Graduate Programs: None available; however, the CTRC has a close association with the University of Texas Health Science Center at San Antonio.

7. Local Accommodations: a) Medical Center Guest House Inn, $49.45/night for a room with 2 beds, stove, refrigerator, phone and cable TV; b) Warren Inn, $115.00/week for studio apt.: no TV or phone; $136/week for 1 bedroom apt.: no TV or phone; $154.00/week for studio apt.: TV and phone; c) Homestead Village, $89.00-$139.00/week for efficiency: TV and phone. All are within walking distance of the CTRC.

8. Transportation: Shuttles or taxi available from the San Antonio International Airport for approximately $10.00/way.

9. Cost of Internships: $800.00 for one week of training/person which will cover laboratory costs, reagents, flow cytometry instrument time, and computer time.

10. Communication:

Steven K. Koester, M.S.
Laboratory Manager/Research Scientist
Flow Cytometry Laboratory Services
Cancer Therapy & Research Center
8122 Data Point Drive, Suite 700
San Antonio, Texas 78229

Phone: (210) 616-5880
FAX: (210) 692-7502 

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If you have suggestions or comments related to the STEP Program in general, problems with this particular page, or you want to know how to be a STEP program lab, send me an email message now jpr@flowcyt.cyto.purdue.edu or contact me at the address below.