Specialty Training & Education Program

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Cell Cycle Analysis
Drug Effects on Cell Cycle Kinetics

Cancer Research Institute (CRI)
New York Medical College
100 Grasslands Road
Elmsford, New York, 10523
Laboratory Profile

Institute Head: Dr. Zbigniew Darzynkiewicz; STEP Director : Dr. Frank Traganos

1. Facilities: Approximately 2,000 sq. foot, three laboratories containing sample preparation and instrument lab, tissue culture facility, molecular biology lab for preparation of gels, and biophysics laboratory.

2. Instrumentation:
Cytometry: Ortho Systems Cytofluorograf II with dual air-cooled lasers, ICP 22 flow cytometer (mercury arc lamp-based), FACScan flow cytometer, and CompuCyte static microscope-based laser scanning cytometer.
Other major items: Zeiss spectrophotometer, Omega titration microcalorimeter, Bio-Rad pulse-field and slab gel electrophoresis apparatus, Nikon Microphot FXA fluorescence microscope with differential interference optics and automatic 35 mm and 4 x 6 cameras, Millipore Cytofluor 2300 automated fluorescence detection system.
Computer and analysis programs: All flow cytometry data analysis systems are IBM PC-based except for Hewlett Packard LYSYS software on the FACSscan flow cytometer. Phoenix Flow Systems Software Acqcyte for acquiring data and Mplus, Multicycle, Multi 2D for data analysis. Software is available for converting HP files to IBM-based files for analysis on Phoenix system programs. Each flow cytometer is connected to its own dedicated computer and laser printer. Other software include SigmaPlot, CorelDraw, and various spreadsheet programs. An HP Scanjet IIc is also available with OCR software for scanning graphics into word processing software (WordPerfect).

3. Specialization Areas: The research at the CRI is focused on apoptosis, cell cycle, and mechanism of action of antitumor drugs. Furthermore, new cytochemical and molecular methods and probes applicable to flow cytometry or static cytometry (CompuCyte) are being developed to assist in this research (See also under Cell Cycle and Drug Effects on Cell Cycle).

4. Special Opportunities: The procedures listed below for analysis of apoptosis or necrosis are routinely used in the laboratory. Most of them were developed in this laboratory. The students are expected to learn the methods of their choice from the following ones:

5. Special Courses Offered: Detection of apoptosis by flow cytometric methods, morphology, and DNA gel electrophoresis. (See also under Cell Cycle and Drug Effects on the Cell Cycle).

6. Graduate Program: None currently available.

7. Local Accommodation: Ramada Inn (200 yds to lab) has a daily rate of $65. Arrangements can be made through the Cancer Research Institute or directly: (914) 592-3300; Eileen O'Donahue (mention N.Y. College for the above discount rate).

8. Transportation: Airports: White Plains, La Guardia, or Kennedy. Buses (Carey) to Grand Central Station ($11.) where one takes Metro North Upper Harlem Line ($10.) to Valhalla or North White Plains. You will be picked up at the station. Taxi from White Plains airport approximately $35.

9. Cost of Internship: A base rate of $150. per day, negotiable for students from disadvantaged countries. Duration of internship, or participation in the course: by arrangement.

10. Communication:

Drs. Frank Traganos
or Zbigniew Darzynkiewicz
Cancer Research Institute
100 Grasslands Road
Elmsford, N.Y. 10523

Phone: (914) 347-2801
FAX: (914) 347-2804
E-mail: darzynk@nymc.edu

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If you have suggestions or comments related to the STEP Program in general, problems with this particular page, or you want to know how to be a STEP program lab, send me an email message now jpr@flowcyt.cyto.purdue.edu or contact me at the address below