This material was originally published in the Purdue Cytometry CD-ROM Series

Flow Cytometry and Microbiology

Combination of flow cytometry and fluorescent probes to determine bacterial viability.

Roger W. Pickup
Institute of Freshwater Ecology
The Ferry House
Far Sawry
Ambleside, UK

Jonathan P. Porter
Department of Genetics and Microbiology
Life Sciences Department
University of Liverpool
Liverpool, L69 3BX, UK

Data Examples

Fluorescent Probes

Porter, J.P., C. Edwards, and R.W. Pickup. 1995. Rapid assessment of physiological status of Escherichia coli using fluorescent probes. Journal of Applied Bacteriology 79:399-408. [reproduced with permission]

Membrane Potential Dyes

Deere, D., J.P. Porter, R.W. Pickup, and C. Edwards. 1995. Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid)trimethine oxonol, (diBA-C4(3)-), for the flow cytometric assessment of bacterial viability. FEMS Microbiology Letters 130:165-170. [reproduced with permission]

Enzymatic Activity

Porter, J., J. Diaper, C. Edwards, and R.W. Pickup. 1995. Direct viability measurements of natural planktonic bacterial communities. Applied and Environmental Microbiology 61:2783-2786. [reproduced with permission]

Acridine Orange
Morgan, J.A.W., K.J. Clark, G. Rhodes, and R.W. Pickup. 1992. Non-culturable Aeromonas salmonicida in lakewater. Microbial Releases 1: 71-78. [reproduced with permission]

Survival Studies
Pickup, R.W., G.Rhodes, R.J. Cobban, and K.J. Clarke. 1996. Postponement of non-culturability in Aeromonas salmonicida. Journal of Fish Diseases 19:65-74. [reproduced with permission]

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