This material was originally published in the Purdue Cytometry CD-ROM Series

Flow Cytometry and Microbiology

Algae Autofluorescence

Hazel M. Davey
Institute of Biological Sciences
University College of Wales, Aberystwyth
Dyfed SY23 3DA, UK


Data Examples

A mixture of Rhinomonas (red algae) and Isochrysis (green algae) was analyzed using a Coulter EPICS ELITE flow cytometer. Fluorescence resulting from excitation with the argon (488 nm) laser was collected using a 575 nm BP filter (PMT3) and a 675 nm BP filter (PMT5). Using autofluorescence, the two populations of algae can easily be distinguished.

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