Data Examples
Bacillus subtilis var. niger (BG), Escherichia coli, Micrococcus luteus and yeast cells were fixed in ethanol and stained with a cocktail of fluorescent dyes (2.5 ug/mL fitc + 50 ug/mL propidium iodide + 40 ug/mL Tinopal CBS-X fluorescent brightener). A Coulter EPICS ELITE flow cytometer was used to analyze the cells immediately after adding the stains.
Figure 1. Fluorescence of a range of microorganisms following staining with FITC. The median fluorescence is lower for BG than for the other organisms but there is significant overlap between BG and Micrococcus luteus. An argon (488 nm) laser was used for excitation.
Figure 2. Fluorescence of a range of microorganisms following staining with propidium iodide. The peak fluorescence is lower for BG than for other organisms but there is significant overlap between BG and E. coli. An argon (488 nm) laser was used for excitation.
Figure 3. Fluorescence of a range of microorganisms following staining with Tinopal CBS-X. The median fluorescence is lower for BG than for the other organisms but there is significant overlap between BG and Micrococcus luteus. A HeCd (325 nm) laser was used for excitation.
Figure 4. Fluorescence histogram overlays of live and oxonol treated Micrococcus luteus cells stained with propidium iodide and analyzed by using a Coulter EPICS ELITE flow cytometer. An argon (488 nm) laser was used for excitation.
email: hlr@aber.ac.uk
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